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National Repository of Grey Literature 2 records found  Search took 0.00 seconds. 
Response of Soil Respiration, Temperature and Moisture to the Harvest of a Sessile Oak Forest\n
 Dařenová, Eva
Forest management, particularly thinning and harvesting measures, has a significant impact on the forest carbon balance. In this study, we investigated the effect of the harvest of a sessile oak forest on soil respiration as a main source of CO2 emissions in forest ecosystems. Soil respiration, temperature and moisture were measured during the two growing seasons, one before and one after the harvest applied on six out of nine studied plots in April 2018. The harvest increased soil temperature by up to 6 °C. Soil moisture did not differ between the plots in 2017, but it was higher in the harvested plots compared to the control in 2018. In the first half of the growing season 2018, the difference between the control and harvested plots ranged between 5 and 100 % in proportion while in the second half the differences decreased only up to 5%. Soil respiration ranged between 1.2 and 3.3 µmol CO2 m-2s-1 during 2017 with no difference between the following variants. In 2018, soil respiration ranged between 1.2 and 7.0 µmol CO2 m-2s-1 and it tended to be higher in the harvested plots compared to the control. The exception was observed shortly after heavy rainfall on 5th September when soil respiration in the control significantly exceeded that in the harvested plots.
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Haplotyping of oak populations by chloroplast sequencing reveals origin and homogeneity of populations
 Vlasák, Josef ; Cvrčková, H. ; Máchová, P.
The aim of this publication is to present new method of DNA isolation from young\noak leaves that enables efficient amplification of trnD-trnT region of chloroplast\nDNA and direct sequencing of crude PCR reaction mixture. Sequence data of trnDtrnT region have been used to examine Quercus robur and Q. petraea populations variability. High discrimination power of this method is demonstrated, comparable with previously used restriction analysis of four similar chloroplast DNA regions. Variable positions in trnT-trnD chloroplast DNA fragment are mapped and their use for oak haplotyping indicated. As an example, 20 Czech Republic oak populations were analyzed and their history, geografical origin and homo/heterogeneity were revealed.
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